MediLytic Urea (GLDH) Reagent Kit

🧪 MediLytic Urea GLDH – 90 ml

Method: GLDH (UV Enzymatic Method)
Category: Biochemistry Reagent
For In-Vitro Diagnostic Use (IVD)

🧬 Test Principle

✔ Urea is hydrolyzed by urease to form ammonia and carbon dioxide
✔ Ammonia reacts with α-ketoglutarate in presence of GLDH (Glutamate Dehydrogenase)
✔ NADH is oxidized to NAD⁺
✔ Decrease in absorbance at 340 nm is measured
✔ Rate of decrease is directly proportional to urea concentration

🧫 Analyte Measured

✔ Urea (Blood Urea)

🩸 Sample Type

✔ Serum
✔ Plasma
✔ Urine

📦 Kit Contents (90 ml Pack)

✔ Reagent R1: 6 × 12 ml
✔ Reagent R2: 6 × 3 ml
✔ Standard: 1 × 3 ml
✔ Number of Tests: 180 tests

🎯 Key Features

✔ UV enzymatic GLDH method
✔ High specificity & accuracy
✔ Interference-free compared to Berthelot method
✔ Suitable for semi-auto & fully auto analyzers
✔ Ideal for precise renal function testing

🧠 Clinical Significance

Urea GLDH testing is important for:

• Kidney function assessment

• Acute & chronic renal failure monitoring

• Dialysis monitoring

• Dehydration evaluation

• ICU & emergency testing

GLDH method provides more specific and reliable results compared to conventional methods.

🌡 Storage & Stability

✔ Store at 2–8°C
✔ Do not freeze
✔ Stable until expiry when stored properly

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