MediLytic Urea (GLDH) 90 ml

Medilytic “Urea (GLDH)” reagent

“Urea (GLDH)” refers to a reagent kit used for quantitative determination of urea (blood urea nitrogen, BUN) in human serum or plasma (and sometimes urine), using the enzymatic Urease–GLDH method

In that method: first urease hydrolyzes urea to ammonia (NH₃) + CO₂; then glutamate dehydrogenase (GLDH) catalyzes a reaction where the ammonia (plus α-ketoglutarate and NADH) is converted to glutamate — and NADH is oxidized to NAD⁺. The decrease in absorbance at 340 nm (because NADH absorbs at 340 nm) is measured. That change in absorbance is proportional to the urea concentration. 

Because it uses a highly specific enzymatic reaction (vs older chemical/colorimetric methods), Urease-GLDH kits are generally considered accurate, reproducible, and suited for automated chemistry analysers.

Measuring blood urea levels (often alongside creatinine) is a standard test to assess kidney function — high urea may indicate impaired kidney filtration, dehydration, or other metabolic issues; low urea may appear in some severe liver diseases. 

It's used in hospital and diagnostic-lab settings for routine biochemistry panels in serum or plasma. 

The “90 ml” refers to the total reagent volume/package size sold by MediLytic. 

Usually such kits are “ready-to-use, liquid-stable” reagents, not requiring reconstitution. 

Store reagent at 2–8 °C (refrigerated) until use.

Mix sample (serum or plasma) with working reagent in defined ratio (depending upon your analyzer’s protocol). 

Incubate at ~ 37 °C (for kits using that temperature) and measure absorbance decrease at 340 nm (or appropriate wavelength). 

Use provided urea standard for calibration.

Use the change in absorbance (ΔA) relative to the standard to calculate urea concentration in sample. 

The test is in vitro diagnostic, and should be used only on human serum or plasma (or urine, if validated) — and only by qualified lab personnel under controlled conditions. 

Reagents must be stored properly (refrigerated, protected from light, no contamination) to maintain stability and accuracy. 

The kit’s validity depends on correct calibration, blank settings, and following the manufacturer protocol exactly.